PES Ultrafiltration Centrifugal Devices
Single-use PES-membrane spin concentrators for rapid concentration, desalting and buffer exchange of LNPs, exosomes, proteins and nucleic acids — 3 volumes, 6 MWCOs, >85–95% typical recovery.
Catalog no. UF-PES-0.5 / 4 / 15 (3K / 5K / 10K / 30K / 50K / 100K) · Ships with certificate of analysis
For Research Use OnlyOverview
At a Glance
Single-use ultrafiltration spin devices with a hydrophilic modified PES (polyethersulfone) membrane. Centrifugal force drives water and small molecules through the membrane into the collection tube while retaining macromolecules — proteins, nucleic acids, exosomes, viral particles and LNPs — in the sample chamber. Concentrate, desalt or buffer-exchange in 15–40 minutes instead of overnight dialysis.
Key Features
- Fast, three-step workflow: load, spin, recover — replaces overnight dialysis bags
- High recovery: >85–95% typical, thanks to a low-binding hydrophilic PES membrane
- Flexible formats: 3 device volumes x 6 MWCOs cover proteins, nucleic acids, exosomes, viral particles and LNPs
- Broad chemical compatibility: pH 1–14; resistant to acids, bases, organic solvents and denaturants
- Universal fit: standard 1.5/2 mL, 15 mL and 50 mL rotors — no dedicated equipment required
- Over-spin protection: physical dead-stop volume prevents the membrane from spinning to dryness; printed graduations for at-a-glance volume reading
Device Specifications
| Parameter | Specification |
|---|---|
| Membrane material | Modified PES, hydrophilic, low protein/nucleic acid binding |
| MWCO options | 3 / 5 / 10 / 30 / 50 / 100 kDa |
| Device volumes | 0.5 / 4 / 15 mL |
| Typical recovery | >85–95% (MWCO- and sample-dependent) |
| Concentration factor | 10–50x |
| pH range | 1–14 |
| Maximum RCF | 4,000 x g (higher force increases membrane breakthrough risk) |
| Sterility | Non-sterile, single-use |
| Intended use | For Research Use Only |
Volume Formats
| Volume | Membrane area |
|---|---|
| 0.5 mL | 0.9 cm2 |
| 4 mL | 3.4 cm2 |
| 15 mL | 9.5 cm2 |
MWCO Selection Guide
| MWCO | Proteins (MW) |
|---|---|
| 3 kDa | ~6–20 kDa |
| 5 kDa | ~15–45 kDa |
| 10 kDa | ~30–90 kDa |
| 30 kDa | ~90–180 kDa |
| 50 kDa | ~150–300 kDa |
| 100 kDa | >300 kDa |
Rule of thumb: select an MWCO at least 3x smaller than your target molecule's molecular weight (e.g., for a 30 kDa target, choose a 10 kDa device) for secure retention and maximum recovery.
Typical Retention & Recovery (15 mL devices, 4,000 x g)
| MWCO | Test sample |
|---|---|
| 3 kDa | Cytochrome c (12.4 kDa) |
| 5 kDa | Lysozyme (14 kDa) |
| 10 kDa | Lysozyme (14 kDa) |
| 30 kDa | BSA (66 kDa) |
| 50 kDa | BSA (66 kDa) |
| 100 kDa | IgG (150 kDa) |
Applications
- LNP / liposome concentration, buffer exchange and removal of free components (untethered lipids, free nucleic acid) ahead of QC or downstream use
- Exosome, viral particle and nanoparticle concentration and purification
- Protein, antibody and enzyme concentration and desalting
- Nucleic acid fragment concentration and clean-up
- Sample prep before HPLC or analytical QC; macromolecule recovery from culture media and lysates
Handling Notes
- Non-sterile, single-use. Do not autoclave or sterilize by UV or alcohol immersion. For sterile workflows, devices may be treated with 70% ethanol before use, or contact us regarding sterile-packaged options.
- As with any ultrafiltration membrane, trace breakthrough can occur — keep the filtrate until recovery in the retentate is confirmed.
- The membrane contains a trace glycerol preservative; rinse with working buffer before processing sensitive samples.
- Balance the rotor carefully and stay within the rated RCF (max 4,000 x g). Spin-down is slower at 4 C than at room temperature.
Research Use Only
For Research Use Only. Not for clinical, diagnostic or therapeutic use in humans.
Ordering & Documentation
Three volumes (0.5 / 4 / 15 mL) x six MWCOs (3–100 kDa). Available configurations, COA, MSDS, ISO documentation, FOB pricing, MOQ and lead time are confirmed per inquiry: Send us an inquiry
| Product | PES Ultrafiltration Centrifugal Devices |
|---|---|
| Catalog no. | UF-PES-0.5 / 4 / 15 (3K / 5K / 10K / 30K / 50K / 100K) |
| Category | LNP Formulation |
| Documentation | Certificate of analysis provided with every lot |
| Intended use | For Research Use Only (RUO). Not for diagnostic or therapeutic use. |
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